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Persian onager (Equus hemionus onager) endometrial explant cryopreservation and in vitro culture.

Authors
  • Thompson, Riley E1
  • Brown, Megan E2
  • Helmick, Kelly3
  • Whitlock, Brian K4
  • Pukazhenthi, Budhan S5
  • 1 University of Tennessee College of Veterinary Medicine, Department of Large Animal Clinical Sciences, 2407 River Drive, Knoxville, TN 37996, USA; Smithsonian Conservation Biology Institute, Center for Species Survival, 1500 Remount Road, Front Royal, VA 22630, USA.
  • 2 Smithsonian Conservation Biology Institute, Center for Species Survival, 1500 Remount Road, Front Royal, VA 22630, USA.
  • 3 Smithsonian Conservation Biology Institute, Department of Conservation Medicine, 1500 Remount Road, Front Royal, VA 22630, USA.
  • 4 University of Tennessee College of Veterinary Medicine, Department of Large Animal Clinical Sciences, 2407 River Drive, Knoxville, TN 37996, USA.
  • 5 Smithsonian Conservation Biology Institute, Center for Species Survival, 1500 Remount Road, Front Royal, VA 22630, USA. Electronic address: [email protected]
Type
Published Article
Journal
Animal reproduction science
Publication Date
Jun 01, 2020
Volume
217
Pages
106459–106459
Identifiers
DOI: 10.1016/j.anireprosci.2020.106459
PMID: 32408971
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Assisted reproduction of endangered equids, such as Persian onagers (Equus hemionus onager), is vital for species conservation. Little is known about Persian onager reproductive functions, including functions of the uterine endometrium. Recently, successful cryopreservation of the domestic mare endometrium was reported, but there is no information on cryo-sensitivity or in vitro culture of endometrial tissues of any non-domestic equid. In the present study, endometrial explants from Persian onagers were cryopreserved and cultured in vitro for 5 days. There was no difference between endometrial explants when 10% and 20% dimethyl sulfoxide (DMSO) was used for cryopreservation. Cell viability and structural integrity were comparable to fresh tissue. Abundance of estrogen receptor-α (ESR1) and progesterone receptor (PGR) mRNA transcript in endometrial explants was less in most treatment groups compared to the fresh tissue control. There was variation in E-cadherin mRNA abundance in endometrial explants among treatment groups with some treatment groups having a lesser abundance compared to the control group. The abundance of Ki67 mRNA transcript of endometrial explants was not different among treatment groups compared to the control group. Results indicate that DMSO is a suitable cryoprotectant for the Persian onager endometrium, and in vitro culture in a liquid-gas interface can maintain Persian onager endometrial explants for as long as 5 days. Findings allow for a greater understanding of reproductive mechanisms in vitro for this endangered species and other domestic equids including donkeys. Published by Elsevier B.V.

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