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Peroxidatic oxidation of benzo[a]pyrene and prostaglandin biosynthesis.

Authors
  • Marnett, L J
  • Reed, G A
Type
Published Article
Journal
Biochemistry
Publication Date
Jul 10, 1979
Volume
18
Issue
14
Pages
2923–2929
Identifiers
PMID: 111706
Source
Medline
License
Unknown

Abstract

The arachidonic acid dependent oxidation of benzo[a]pyrene to a mixture of 3,6-, 1,6-, and 6,12-quinones has been studied by using enzyme preparations from sheep seminal vesicles. Maximal oxidation is observed at 100 microM benzo[a]pyrene and 150 microM arachidonic acid. The arachidonic acid dependent oxidation is peroxidatic and utilizes prostaglandin G2 (PGG2), generated in situ from arachidonate, as the hydroperoxide substrate. 15-Hydroperoxy-5,8,11,13-eicosatetraenoic acid is equivalent to PGG2 as a hydroperoxide substrate, but hydrogen peroxide, cumene hydroperoxide, and tert-butyl hydroperoxide are much poorer substrates. Arachidonic acid dependent benzo[a]pyrene oxidation by microsomal and solubilized enzyme preparations is markedly.

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