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Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

Authors
  • Xie, Shijun1, 2
  • Li, Fuhua1, 3
  • Zhang, Xiaojun1
  • Zhang, Jiquan1
  • Xiang, Jianhai1, 3
  • 1 Chinese Academy of Sciences, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Qingdao, 266071, China , Qingdao (China)
  • 2 University of Chinese Academy of Sciences, Beijing, 100049, China , Beijing (China)
  • 3 Qingdao National Laboratory for Marine Science and Technology, Laboratory for Marine Biology and Biotechnology, Qingdao, 266071, China , Qingdao (China)
Type
Published Article
Journal
Chinese Journal of Oceanology and Limnology
Publisher
Science Press
Publication Date
May 13, 2017
Volume
35
Issue
6
Pages
1524–1530
Identifiers
DOI: 10.1007/s00343-017-6109-2
Source
Springer Nature
Keywords
License
Yellow

Abstract

The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at ~27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

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