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Performance of six SARS-CoV-2 RNA detection systems in symptomatic and asymptomatic pediatric and maternal patients

Authors
  • Brown, Cameron A
  • Amerson-Brown, Megan H
  • Rahman, Aliza
  • Webb, Charity R
  • Singh, Ila R
  • Dunn, James J
Type
Published Article
Journal
Future Virology
Publisher
Future Medicine
Publication Date
Nov 01, 2021
Identifiers
DOI: 10.2217/fvl-2021-0311
PMID: 35027939
PMCID: PMC8734120
Source
PubMed Central
Keywords
Disciplines
  • Short Communication
License
Unknown

Abstract

Aim: This study evaluated the real-world performance of six test systems for detection of SARS-CoV-2 in 138 pediatric and 110 adult maternal patients. Materials & methods: Nasopharyngeal swabs were tested directly using the Aptima™ SARS-CoV-2 (Aptima) and Simplexa™ COVID-19 Direct (Simplexa), and with Altona RealStar® RT-PCR and CDC RT-PCR with nucleic acid extracted on the Roche® MagNA Pure 96 (Altona-MP96) or bioMérieux EMAG® (Altona-EMAG). Results/Conclusion: Overall percent-positive and percent-negative agreements among the six test systems were, respectively: Aptima: 94.8 and 100%; Altona-MP96: 96.5 and 99.3%; CDC-MP96: 100 and 99.3%; Altona-EMAG: 86.1 and 100%; CDC-EMAG: 98.2 and 100%; Simplexa: 87 and 99.2%. The six test systems showed agreement ranging from 92.7 (κ = 0.85) to 98.8% (κ = 0.98).

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