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Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory.

Authors
  • Buitrago, M J
  • Bernal-Martinez, L
  • Castelli, M V
  • Rodriguez-Tudela, J L
  • Cuenca-Estrella, M
Type
Published Article
Journal
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Publication Date
May 01, 2014
Volume
52
Issue
5
Pages
1737–1740
Identifiers
DOI: 10.1128/JCM.00328-14
PMID: 24574295
Source
Medline
License
Unknown

Abstract

A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.

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