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Perforin is recaptured by natural killer cells following target cells stimulation for cytotoxicity.

Authors
  • Wang, Lei
  • Sun, Rulin
  • Li, Pan
  • Han, Yang
  • Xiong, Ping
  • Xu, Yong
  • Fang, Min
  • Tan, Zheng
  • Zheng, Fang
  • Gong, Feili
Type
Published Article
Journal
Cell Biology International
Publisher
Wiley (John Wiley & Sons)
Publication Date
Feb 01, 2012
Volume
36
Issue
2
Pages
223–228
Identifiers
DOI: 10.1042/CBI20110242
PMID: 21981014
Source
Medline
License
Unknown

Abstract

When encountering target cells, NK (natural killer) cells exocytose Pfn (perforin) and granzyme B to kill challengers. We previously reported that granzyme B is recycled and reused by NK cells via clathrin-dependent endocytosis. However, whether Pfn, a main secretory vesicle content, indispensible to granzyme B killing, undergoes endocytosis remains unknown. We demonstrate that Pfn is recaptured by early endosomes of NK cells via a clathrin-dependent endocytosis after target cell stimulation. Inhibition of clathrin-dependent endocytosis significantly attenuated the cytotoxicity of NK cells. The data suggest that the recovery of Pfn contributes to the cytotoxicity of NK cells. The assay of endocytosis of lytic molecule presents a particular focus for exploring the mechanism of abnormal cytotoxicity of NK cells.

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