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Percutaneous Sampling of Local Biomolecule Gradients Across Coronary Artery Atherosclerotic Plaques

Authors
  • West, Nick E.J.
  • Corrigan, Joseph P.
  • Owen, Richard H.G.
  • Hoole, Stephen P.
  • Brown, Adam J.
  • Blatcher, Stephen
  • Newby, Andrew C.
Publication Date
Dec 25, 2017
Source
PubMed Central
Keywords
Language
English
License
Unknown
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Abstract

A percutaneous catheter device, the Liquid Biopsy System, was developed to sample the unstirred boundary layer of blood upstream and downstream of intact and disrupted human coronary atherosclerotic plaques. Using multiplexed proximity extension assays, release of 20 biomolecules was simultaneously detected in samples taken across plaques before balloon angioplasty, including the soluble form of the endothelial lectin-like oxidized LDL receptor. Additional biomolecules, including matrix metalloproteinase-12, were released after plaque disruption with angioplasty. These experiments demonstrate the power of the Liquid Biopsy System to yield new scientific insights and its ultimate potential to generate new biomarkers and surrogate endpoints for clinical trials.

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