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A Penicillium freii gene that is highly similar to the beta-ketoacyl synthase domain of polyketide synthase genes from other fungi.

Authors
Type
Published Article
Journal
Letters in applied microbiology
Publication Date
Volume
25
Issue
3
Pages
197–201
Identifiers
PMID: 9351263
Source
Medline
License
Unknown

Abstract

A Penicillium freii DNA fragment with similarity to the beta-keto-acyl synthase motif of the P. griseofulvum 6-methylsalicylic acid synthase encoding gene (MSAS) was identified by screening a cosmid library using a part of MSAS as the probe. Two exons of 93 and 849 bp, encoding a predicted polypeptide of 314 amino acids, and a molecular mass of 33.4 kDa, were identified (PfKS). PfKS was transcribed as a 1.6 kbp messenger. A region corresponding to the MSAS gene encoding essential enzyme activities for the assembly of a polyketide chain was absent in PfKS. Gene disruption experiments in P. freii with a truncated version of PfKS did not result in the detection of homologous integration events.

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