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PEG-modified glucose oxidase immobilized on a PVA cryogel membrane for amperometric biosensor applications.

Authors
  • Doretti, L
  • Ferrara, D
  • Gattolin, P
  • Lora, S
  • Schiavon, F
  • Veronese, F M
Type
Published Article
Journal
Talanta
Publisher
Elsevier
Publication Date
Mar 01, 1998
Volume
45
Issue
5
Pages
891–898
Identifiers
PMID: 18967075
Source
Medline
License
Unknown

Abstract

Poly(ethylene glycol)-modified glucose oxidase was immobilized in a poly(vinyl alcohol) cryogel membrane, obtained by a freezing-thawing cyclic process, to obtain a suitable amperometric glucose sensor. The covalent linkage between PEG and GOD molecule improved the physical immobilization of enzyme in the polymeric matrix, by decreasing its loss in time. Sensor behaviour was evaluated electrochemically with a hydrogen peroxide electrode. The glucose content in standard solutions was determined and linear calibration curves in the 5x10(-5)-5x10(-3) mol l(-1) range were obtained. The kinetic parameters in the immobilized system were evaluated and analytical characteristics of sensor, including stability and influence of pH and temperature, were determined.

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