Affordable Access

deepdyve-link
Publisher Website

PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations

Authors
  • Caillet-Saguy, Célia
  • Brûlé, Sébastien
  • Wolff, Nicolas
  • Raynal, Bertrand
Publication Date
May 21, 2021
Identifiers
DOI: 10.1007/978-1-0716-1166-1_6
OAI: oai:HAL:hal-03234405v1
Source
HAL-INRIA
Keywords
Language
English
License
Unknown
External links

Abstract

PDZ domains are small globular domains involved in protein–protein interactions. They participate in a wide range of critical cellular processes. These domains, very abundant in the human proteome, are widely studied by high-throughput interactomics approaches and by biophysical and structural methods. However, the quality of the results is strongly related to the optimal folding and solubility of the domains. We provide here a detailed description of protocols for a strict quality assessment of the PDZ constructs. We describe appropriate experimental approaches that have been selected to overcome the small size of such domains to check the purity, identity, homogeneity, stability, and folding of samples.

Report this publication

Statistics

Seen <100 times