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PdWND3A, a wood-associated NAC domain-containing protein, affects lignin biosynthesis and composition in Populus

  • Yang, Yongil1
  • Yoo, Chang Geun1, 2, 3
  • Rottmann, William4
  • Winkeler, Kimberly A.4
  • Collins, Cassandra M.4
  • Gunter, Lee E.1, 2
  • Jawdy, Sara S.1, 2
  • Yang, Xiaohan1, 2
  • Pu, Yunqiao1, 2, 3
  • Ragauskas, Arthur J.1, 2, 3, 5
  • Tuskan, Gerald A.1, 2
  • Chen, Jin-Gui1, 2
  • 1 Oak Ridge National Laboratory, BioEnergy Science Center and Biosciences Division, Oak Ridge, TN, 37831, USA , Oak Ridge (United States)
  • 2 Oak Ridge National Laboratory, Center for Bioenergy Innovation, Oak Ridge, TN, 37831, USA , Oak Ridge (United States)
  • 3 Oak Ridge National Laboratory, UT-ORNL Joint Institute for Biological Science, Oak Ridge, TN, 37831, USA , Oak Ridge (United States)
  • 4 ArborGen Inc., Ridgeville, SC, 29472, USA , Ridgeville (United States)
  • 5 University of Tennessee, Department of Chemical and Biomolecular Engineering & Department of Forestry, Wildlife, and Fisheries, Knoxville, TN, 37996, USA , Knoxville (United States)
Published Article
BMC Plant Biology
Springer (Biomed Central Ltd.)
Publication Date
Nov 11, 2019
DOI: 10.1186/s12870-019-2111-5
Springer Nature


BackgroundPlant secondary cell wall is a renewable feedstock for biofuels and biomaterials production. Arabidopsis VASCULAR-RELATED NAC DOMAIN (VND) has been demonstrated to be a key transcription factor regulating secondary cell wall biosynthesis. However, less is known about its role in the woody species.ResultsHere we report the functional characterization of Populus deltoides WOOD-ASSOCIATED NAC DOMAIN protein 3 (PdWND3A), a sequence homolog of Arabidopsis VND4 and VND5 that are members of transcription factor networks regulating secondary cell wall biosynthesis. PdWND3A was expressed at higher level in the xylem than in other tissues. The stem tissues of transgenic P. deltoides overexpressing PdWND3A (OXPdWND3A) contained more vessel cells than that of wild-type plants. Furthermore, lignin content and lignin monomer syringyl and guaiacyl (S/G) ratio were higher in OXPdWND3A transgenic plants than in wild-type plants. Consistent with these observations, the expression of FERULATE 5-HYDROXYLASE1 (F5H1), encoding an enzyme involved in the biosynthesis of sinapyl alcohol (S unit monolignol), was elevated in OXPdWND3A transgenic plants. Saccharification analysis indicated that the rate of sugar release was reduced in the transgenic plants. In addition, OXPdWND3A transgenic plants produced lower amounts of biomass than wild-type plants.ConclusionsPdWND3A affects lignin biosynthesis and composition and negatively impacts sugar release and biomass production.

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