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PCV2 Replicase Transcripts in Infected Porcine Kidney (PK15) Cells

Authors
  • Bratanich, A.C.1
  • Blanchetot, A.2
  • 1 University of Buenos Aires, Department of Virology, School of Veterinary Science, Chorroarin 280, Buenos Aires, 1427, Argentina , Buenos Aires
  • 2 Marshall University, IST Department, Huntington, WV, 257 55, USA , Huntington
Type
Published Article
Journal
Virus Genes
Publisher
Springer-Verlag
Publication Date
Dec 01, 2002
Volume
25
Issue
3
Pages
323–328
Identifiers
DOI: 10.1023/A:1020940411267
Source
Springer Nature
Keywords
License
Yellow

Abstract

The Circoviridae family includes small viruses containing circular single strand DNA. There are two circoviruses described in pigs. Porcine Circovirus 2 (PCV2) has been linked to a disease termed Postweaning Multisystemic Wasting Syndrome (PMWS) whereas a close relative of this virus, termed PCV type 1 (PCV1) has been considered a non-pathogenic contaminant of the PK15 cell line. Rolling circle replication of small DNA viruses like circoviruses is mediated by a replicase (Rep) gene that is transcribed into different mRNAs by an alternative splicing mechanism. In PCV1, two transcripts Rep and Rep′ implicated in DNA replication, have been identified from infected cells. These transcripts are the product of a single gene with Rep′ having a different carboxyl end than Rep. Sequence comparison shows that there is a high degree of homology for the Rep gene in both viruses. In this article, we have identified three different transcripts by RT-PCR in PCV2 infected PK15 cells. Two of them have homology with their PCV1 counterparts and the third transcript, termed Rep″, corresponds to another spliced version of the Rep gene. This transcript, unique to PCV2, encodes for a protein of 80 aminoacids in frame with the Rep sequence.

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