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pCADD: SNV prioritisation in Sus scrofa

Authors
  • Groß, Christian1, 2
  • Derks, Martijn3
  • Megens, Hendrik-Jan3
  • Bosse, Mirte3
  • Groenen, Martien A. M.3
  • Reinders, Marcel1
  • de Ridder, Dick2
  • 1 University of Technology Delft, Delft, 2600GA, The Netherlands , Delft (Netherlands)
  • 2 Wageningen University & Research, Wageningen, 6708 PB, The Netherlands , Wageningen (Netherlands)
  • 3 Wageningen University & Research, Wageningen, The Netherlands , Wageningen (Netherlands)
Type
Published Article
Journal
Genetics Selection Evolution
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Feb 07, 2020
Volume
52
Issue
1
Identifiers
DOI: 10.1186/s12711-020-0528-9
Source
Springer Nature
License
Green

Abstract

BackgroundIn animal breeding, identification of causative genetic variants is of major importance and high economical value. Usually, the number of candidate variants exceeds the number of variants that can be validated. One way of prioritizing probable candidates is by evaluating their potential to have a deleterious effect, e.g. by predicting their consequence. Due to experimental difficulties to evaluate variants that do not cause an amino-acid substitution, other prioritization methods are needed. For human genomes, the prediction of deleterious genomic variants has taken a step forward with the introduction of the combined annotation dependent depletion (CADD) method. In theory, this approach can be applied to any species. Here, we present pCADD (p for pig), a model to score single nucleotide variants (SNVs) in pig genomes.ResultsTo evaluate whether pCADD captures sites with biological meaning, we used transcripts from miRNAs and introns, sequences from genes that are specific for a particular tissue, and the different sites of codons, to test how well pCADD scores differentiate between functional and non-functional elements. Furthermore, we conducted an assessment of examples of non-coding and coding SNVs, which are causal for changes in phenotypes. Our results show that pCADD scores discriminate between functional and non-functional sequences and prioritize functional SNVs, and that pCADD is able to score the different positions in a codon relative to their redundancy. Taken together, these results indicate that based on pCADD scores, regions with biological relevance can be identified and distinguished according to their rate of adaptation.ConclusionsWe present the ability of pCADD to prioritize SNVs in the pig genome with respect to their putative deleteriousness, in accordance to the biological significance of the region in which they are located. We created scores for all possible SNVs, coding and non-coding, for all autosomes and the X chromosome of the pig reference sequence Sscrofa11.1, proposing a toolbox to prioritize variants and evaluate sequences to highlight new sites of interest to explain biological functions that are relevant to animal breeding.

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