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Pathway engineering for beta-carotene and carotenoid biosynthesis in Y. lipolytica.

Authors
  • Pesantes-Munoz, M
  • Ledesma-Amaro, R
Publication Date
Apr 14, 2021
Source
UPCommons. Portal del coneixement obert de la UPC
Keywords
License
Unknown

Abstract

β-carotene is an increasingly sought-after organic pigment with antioxidant properties and a vitamin precursor. Yarrowia lipolytica, though unable to naturally synthesize carotenoids, can produce high amounts of the precursor acetyl-CoA making it a promising host for metabolic engineering towards novel biotechnological production of carotenoids. Here, we describe a synthetic biology methodology for Y. Lipolytica metabolic engineering based on Golden Gate DNA assembly for the generation of a multigene cassette, subsequent transformation enabling β-carotene biosynthesis, and quantification of the compound.

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