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Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally-infected does.

Authors
  • Mazlan, Mazlina1
  • Khairani-Bejo, Siti1
  • Hamzah, Hazilawati1
  • Nasruddin, Nurrul Shaqinah2
  • Salleh, Annas3
  • Zamri-Saad, Mohd3
  • 1 Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang, Malaysia; , (Malaysia)
  • 2 Faculty of Dentistry, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, Kuala Lumpur, Malaysia; , (Malaysia)
  • 3 Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia. , (Malaysia)
Type
Published Article
Journal
The veterinary quarterly
Publication Date
Dec 21, 2020
Pages
1–15
Identifiers
DOI: 10.1080/01652176.2020.1867328
PMID: 33349157
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Background: Brucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from wildlife such as ibex, deer and wild buffaloes. Objective: To describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does. Methods: Twelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 μL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100μL of an inoculum containing 109 CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination. Results: Bilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs. Conclusion: Vertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue.

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