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PathoGene: a pathogen coding sequence discovery and analysis resource.

Authors
  • Ng, Kar-wai1
  • Lawson, Jonathan
  • Garner, Harold R
  • 1 The University of Texas Southwestern Medical Center, Dallas, TX 75390-8591, USA.
Type
Report
Journal
BioTechniques
Publication Date
Aug 01, 2004
Volume
37
Issue
2
Identifiers
PMID: 15335212
Source
Medline
License
Unknown

Abstract

PathoGene is a web-based resource that streamlines the process of predicting genes in microorganisms and designs PCR primers for amplification to facilitate sequence analysis and experimentation. PathoGene currently supports primer design for every complete microbial, viral, and fungal genome as cataloged in GenBank by the National Center for Biotechnology Information (NCBI; http://www.ncbi.nlm.nih.gov/). The resulting primers can then be subjected to a stand-alone Basic Local Alignment Search Tool (BLAST) system called PathoBLAST in which the predicted PCR product and/or primers can be compared against the genome of interest or a similar genome to find related genes or estimate primer quality.

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