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Partial purification from bovine pulmonary tissue of a protein capable of inhibiting in vivo DNA synthesis in mouse pulmonary cells.

Authors
  • Marcsek, Z
  • Menyhárt, J
Type
Published Article
Journal
Acta physiologica Academiae Scientiarum Hungaricae
Publication Date
Jan 01, 1982
Volume
59
Issue
4
Pages
349–358
Identifiers
PMID: 7170987
Source
Medline
Language
English
License
Unknown

Abstract

This paper reports on partial purification and characterization of a natural (endogenous) factor capable of inhibiting in vivo DNA synthesis in mouse pneumocytes in a tissue-specific manner. By using a combination of ultrafiltration and various chromatographic techniques, the active agent has been partially purified from aqueous extracts of both bovine and rat pulmonary tissue. The factor responsible for the observed effect was found to be a heat labile compound, most likely a protein of approx. 40 000 molecular weight. Its chemical and physicochemical properties determined so far, and also the manner of its biological action implies that this lung tissue derived agent might be an endogenous proliferation inhibitor with a chalone-like character operating in the pulmonary epithelial cells.

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