Partial purification of 2,3-oxidosqualene-lanosterol cyclase from hog-liver. Evidence for a functional thiol residue.
Laboratoire de Chimie Enzymatique, CNRS UA 1182, Strasbourg, France.
- Published Article
Biochemical and biophysical research communications
- Publication Date
Mar 30, 1988
2,3-Oxidosqualene-lanosterol cyclase is an intrinsic microsomal protein which can be solubilized by ionic (deoxycholate) and nonionic (emulphogene) detergents with good yields. The hog-liver microsomal cyclase was purified approximately 140-fold by chromatography on DEAE-cellulose and hydroxylapatite. The partially purified enzyme was inactivated by N-ethylmaleimide, following pseudo-first order kinetics, indicating that a cysteine residue is essential for activity.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/3355560