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Parkia platycephala lectin enhances the antibiotic activity against multi-resistant bacterial strains and inhibits the development of Haemonchus contortus.

Authors
  • Silva, Romerio R S1
  • Silva, Carolina R2
  • Santos, Valdenice F1
  • Barbosa, Cristina R S3
  • Muniz, Debora F3
  • Santos, Ana L E1
  • Santos, Maria H C1
  • Rocha, Bruno A M4
  • Batista, Karla L R1
  • Costa-Júnior, Livio M2
  • Coutinho, Henrique D M3
  • Teixeira, Claudener S5
  • 1 Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil. , (Brazil)
  • 2 Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil. , (Brazil)
  • 3 Departamento de Química Biológica, Universidade Regional do Cariri, Crato, Ceará, Brazil. , (Brazil)
  • 4 Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Ceará, Brazil. , (Brazil)
  • 5 Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Microbial Pathogenesis
Publisher
Elsevier
Publication Date
Oct 01, 2019
Volume
135
Pages
103629–103629
Identifiers
DOI: 10.1016/j.micpath.2019.103629
PMID: 31325571
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lectins have been studied in the past few years as an alternative to inhibit the development of pathogenic bacteria and gastrointestinal nematodes of small ruminants. The development of new antibacterial and anthelmintic compounds is necessary owing to the increase in drug resistance among important pathogens. Therefore, this study aimed to evaluate the capacity of a glucose/mannose-binding lectin from Parkia platycephala seeds (PPL) to inhibit the development of Haemonchus contortus and to modulate antibiotic activity against multi-resistant bacterial strains, thereby confirming its efficacy when used in combination with gentamicin. PPL at the concentration of 1.2 mg/mL did not show inhibitory activity on H. contortus in the egg hatch test or the exsheathment assay. However, it did show significant inhibition of H. contortus larval development with an IC50 of 0.31 mg/mL. The minimum inhibitory concentration (MIC) obtained for PPL against all tested bacterial strains was not clinically relevant (MIC ≥ 1024 μg/mL). However, when PPL was combined with gentamicin, a significant increase in antibiotic activity was observed against S. aureus and E.coli multi-resistant strains. The inhibition of hemagglutinating activity by gentamicin (MIC = 50 mM) revealed that it may be interacting with the carbohydrate-binding site of PPL. It is this interaction between the antibiotic and lectin carbohydrate-binding site that may be responsible for the enhanced activity of gentamicin against multi-resistant strains. It can be concluded that PPL showed selective anthelmintic effect, inhibiting the development of H. contortus larvae and that it increased the effect of the antibiotic gentamicin against multi-resistant bacterial strains, thus constituting a potential therapeutic resource against resistant bacterial strains and H. contortus. Copyright © 2019 Elsevier Ltd. All rights reserved.

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