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Parallel [TG(GA)3]n-homoduplexes/thioflavin T: an intense and stable fluorescent indicator for label-free biosensing.

Authors
  • Liu, Qiang1
  • Jing, Shaochun
  • Liu, Mei
  • Jin, Yan
  • Li, Baoxin
  • 1 School of Chemistry & Chemical Engineering, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, China. [email protected] , (China)
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Dec 16, 2019
Volume
145
Issue
1
Pages
286–294
Identifiers
DOI: 10.1039/c9an01856e
PMID: 31750449
Source
Medline
Language
English
License
Unknown

Abstract

Different from the classical antiparallel DNA double-stranded structure, parallel DNA duplexes possess unique structures and potential biological functions. In this work, we found that the parallel DNA homoduplex from the [TG(GA)3]n sequence ([TG(GA)3]n-dsDNA) can dramatically enhance the fluorescence of thioflavin T (ThT), and the fluorescence enhancement is proportional to the number (n) of TG(GA)3 units in [TG(GA)3]n. Compared with the traditional G-quadruplex/ThT system, [TG(GA)3]n/ThT showed more stable and stronger fluorescence emission. In addition, coupled with an isothermal exponential amplification reaction, [TG(GA)3]3/ThT was used as a label-free fluorescent probe to detect microRNA, and the [TG(GA)3]3/ThT probe exhibited higher sensitivity than the G-quadruplex/ThT probe. This work provides a new paradigm to design label-free fluorescent biosensing/imaging systems.

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