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Paraformaldehyde fixation of Drosophila testes.

Authors
  • Bonaccorsi, Silvia
  • Giansanti, Maria G
  • Cenci, Giovanni
  • Gatti, Maurizio
Type
Published Article
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory
Publication Date
Jan 01, 2012
Volume
2012
Issue
1
Pages
102–104
Identifiers
DOI: 10.1101/pdb.prot067330
PMID: 22194254
Source
Medline
License
Unknown

Abstract

This protocol describes paraformaldehyde fixation of Drosophila testes. The procedure preserves F-actin-containing structures because it does not involve the use of methanol. Thus, it is particularly suitable for contractile ring and fusome visualization. However, it does not preserve cell morphology and microtubules as efficiently as methanol-acetone fixation and does not permit clear detection of the Y loops.

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