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Paper-based chemiluminescence immunodevice for the carcinoembryonic antigen by employing multi-enzyme carbon nanosphere signal enhancement

Authors
  • Chen, Ying1
  • Chu, Weiru1
  • Liu, Wei1
  • Guo, Xiaoyan1
  • Jin, Yan1
  • Li, Baoxin1
  • 1 Shaanxi Normal University, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Xi’an, 710062, China , Xi’an (China)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Feb 19, 2018
Volume
185
Issue
3
Identifiers
DOI: 10.1007/s00604-018-2726-5
Source
Springer Nature
Keywords
License
Yellow

Abstract

A paper-based chemiluminescence (CL) immunodevice is described for sensitive determination of the carcinoembryonic antigen (CEA). Capture antibody (Ab1) was first immobilized on the paper-based chip after plasma treatment of the paper surface. Next, carbon nanospheres functionalized with horse radish peroxidase (HRP) were attached as a signal antibody label. Strongly enhanced CL was achieved from the HRP catalyzed luminol/H2O2 CL system when using a sandwich immunoassay format. CEA can be quantified with a lower detection limit of 3 pg·mL−1. The method displays an almost 10-fold increased sensitivity in comparison to a commercial Ab2-HRP based kit. Conceivably, this sensitive method may also be extended by designing sensitive assays for other biomarkers of interest in point-of-care diagnostics. Graphical abstractSchematic of a paper-based chemiluminescence (CL) immunodevice for the determination of the carcinoembryonic antigen (CEA) by applying a multi-enzyme carbon nanosphere signal enhancement strategy.

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