Affordable Access

deepdyve-link
Publisher Website

Pannexin 1, a large-pore membrane channel, contributes to hypotonicity-induced ATP release in Schwann cells.

Authors
  • Wei, Zhong-Ya1
  • Qu, Hui-Lin1
  • Dai, Yu-Juan2
  • Wang, Qian1
  • Ling, Zhuo-Min2
  • Su, Wen-Feng1
  • Zhao, Ya-Yu1
  • Shen, Wei-Xing2
  • Chen, Gang3
  • 1 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China. , (China)
  • 2 Medical School of Nantong University, Nantong, Jiangsu Province, China. , (China)
  • 3 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University; Medical School of Nantong University; Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China. , (China)
Type
Published Article
Journal
Neural Regeneration Research
Publisher
Medknow Publications
Publication Date
May 01, 2021
Volume
16
Issue
5
Pages
899–904
Identifiers
DOI: 10.4103/1673-5374.290911
PMID: 33229726
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Pannexin 1 (Panx 1), as a large-pore membrane channel, is highly permeable to ATP and other signaling molecules. Previous studies have demonstrated the expression of Panx 1 in the nervous system, including astrocytes, microglia, and neurons. However, the distribution and function of Panx 1 in the peripheral nervous system are not clear. Blocking the function of Panx 1 pharmacologically (carbenoxolone and probenecid) or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release. Treatment of Schwann cells with a Ras homolog family member (Rho) GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents, such as nocodazole or cytochalasin D, revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton. These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement. This study was approved by the Animal Ethics Committee of Nantong University, China (No. S20180806-002) on August 5, 2018.

Report this publication

Statistics

Seen <100 times