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Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors
  • B Hohn
  • K Murray
Publication Date
Aug 01, 1977
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

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