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Oxygen-dependent delayed fluorescence of protoporphyrin IX measured in the stomach and duodenum during upper gastrointestinal endoscopy.

Authors
  • van Dijk, Louisa J D1, 2
  • Ubbink, Rinse3
  • Terlouw, Luke G1, 2
  • van Noord, Desirée1, 4
  • Mik, Egbert G3
  • Bruno, Marco J1
  • 1 Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands. , (Netherlands)
  • 2 Department of Radiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands. , (Netherlands)
  • 3 Department of Anesthesiology, Laboratory for Experimental Anesthesiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands. , (Netherlands)
  • 4 Department of Gastroenterology and Hepatology, Franciscus Gasthuis and Vlietland, Rotterdam, The Netherlands. , (Netherlands)
Type
Published Article
Journal
Journal of Biophotonics
Publisher
Wiley (John Wiley & Sons)
Publication Date
Oct 01, 2019
Volume
12
Issue
10
Identifiers
DOI: 10.1002/jbio.201900025
PMID: 31140739
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) is a method used to measure oxygen (PO2 ) in human cells. The aim of this study was to assess the technical feasibility and safety of measuring oxygen-dependent delayed fluorescence of 5-aminolevulinic acid (ALA)-induced PpIX during upper gastrointestinal (GI) endoscopy. Endoscopic delayed fluorescence measurements were performed 4 hours after oral administration of ALA in healthy volunteers. The ALA dose administered was 0, 1, 5 or 20 mg/kg. Measurements were performed at three mucosal spots in the gastric antrum, duodenal bulb and descending duodenum with the catheter above the mucosa and while applying pressure to induce local ischemia and monitor mitochondrial respiration. During two endoscopies, measurements were performed both before and after intravenous administration of butylscopolamine. Delayed fluorescence measurements were successfully performed during all 10 upper GI endoscopies. ALA dose of 5 mg/kg showed adequate signal-to-noise ratio (SNR) values >20 without side effects. All pressure measurements showed significant prolongation of delayed fluorescence lifetime compared to measurements performed without pressure (P < .001). Measurements before and after administration of butylscopolamine did not differ significantly in the duodenal bulb and descending duodenum. Measurements of oxygen-dependent delayed fluorescence of ALA-induced PpIX in the GI tract during upper GI endoscopy are technically feasible and safe. © 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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