Affordable Access

deepdyve-link
Publisher Website

OxLDL alterations in endothelial cell membrane dynamics leads to changes in vesicle trafficking and increases cell susceptibility to injury.

Authors
  • do Couto, Natália Fernanda1
  • Rezende, Luisa1
  • Fernandes-Braga, Weslley2
  • Alves, Ana Paula3
  • Agero, Ubirajara3
  • Alvarez-Leite, Jacqueline2
  • Damasceno, Nágila Raquel Teixeira4
  • Castro-Gomes, Thiago5
  • Andrade, Luciana O6
  • 1 Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. , (Brazil)
  • 2 Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. , (Brazil)
  • 3 Department of Physics, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. , (Brazil)
  • 4 Department of Nutrition, University of São Paulo, São Paulo, SP, Brazil. , (Brazil)
  • 5 Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. , (Brazil)
  • 6 Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Biochimica et biophysica acta. Biomembranes
Publication Date
Dec 05, 2019
Pages
183139–183139
Identifiers
DOI: 10.1016/j.bbamem.2019.183139
PMID: 31812625
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Plasma membrane repair (PMR) is an important process for cell homeostasis, especially for cells under constant physical stress. Repair involves a sequence of Ca2+-dependent events, including lysosomal exocytosis and subsequent compensatory endocytosis. Cholesterol sequestration from plasma membrane causes actin cytoskeleton reorganization and polymerization, increasing cell stiffness, which leads to exocytosis and reduction of a peripheral pool of lysosomes involved in PMR. These changes in mechanical properties are similar to those observed in cells exposed to oxidized Low Density Lipoprotein (oxLDL), a key molecule during atherosclerosis development. Using a human umbilical vein endothelial cell line (EAhY926) we evaluated the influence of mechanical modulation induced by oxLDL in PMR and its effect in endothelial fragility. Similar to MβCD (a drug capable of sequestering cholesterol) treatment, oxLDL exposure led to actin reorganization and de novo polymerization, as well as an increase in cell rigidity and lysosomal exocytosis. Additionally, for both MβCD and oxLDL treated cells, there was an initial increase in endocytic events, likely triggered by the peak of exocytosis induced by both treatments. However, no further endocytic events were observed, suggesting that constitutive endocytosis is blocked upon treatment and that the reorganized cytoskeleton function as a mechanical barrier to membrane traffic. Finally, the increase in cell rigidity renders cells more prone to mechanical injury. Together, these data show that mechanical modulation induced by oxLDL exposure not only alters membrane traffic in cells, but also makes them more susceptible to mechanical injury, which may likely contribute to the initial steps of atherosclerosis development. Copyright © 2019. Published by Elsevier B.V.

Report this publication

Statistics

Seen <100 times