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Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice

Authors
  • Zhang, Jin1, 2
  • Dong, Juan1
  • Qin, Weibing3
  • Cao, Congcong1
  • Wen, Yujiao1
  • Tang, Yunge3
  • Yuan, Shuiqiao1, 4
  • 1 Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China , Wuhan (China)
  • 2 College of Animal Science and Technology, Northwest A&F University, Yangling, People’s Republic of China , Yangling (China)
  • 3 NHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou, People’s Republic of China , Guangzhou (China)
  • 4 Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, People’s Republic of China , Shenzhen (China)
Type
Published Article
Journal
Reproductive Biology and Endocrinology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Nov 23, 2019
Volume
17
Issue
1
Identifiers
DOI: 10.1186/s12958-019-0542-3
Source
Springer Nature
Keywords
License
Green

Abstract

Ovol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Here, we firstly examined the mRNA and protein levels of Ovol2 in developing mouse testes by RT-qPCR and western blot and found that both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its higher level at adult testis. Further testicular immuno-staining revealed that OVOL2 is highly expressed in the spermatogonia, spermatocytes and round spermatids. Interestingly, our conditional ovol2 knockout mouse model show that loss of ovol2 in embryonic germ cells does not affect fecundity in mice. Our data also show that Ovol1 may have compensated for the loss of Ovol2 functions in germ cells. Overall, our data indicate that ovol2 is dispensable for germ cell development and spermatogenesis.

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