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Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants

Authors
  • Zhang, Langlang1, 2
  • Zhao, Tingting1
  • Sun, Xiaoming1, 3
  • Wang, Yi3
  • Du, Chang1
  • Zhu, Zhenfei1, 2
  • Gichuki, Duncan Kiragu1, 2
  • Wang, Qingfeng1
  • Li, Shaohua3
  • Xin, Haiping1
  • 1 Chinese Academy of Sciences, Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Wuhan, 430074, People’s Republic of China , Wuhan (China)
  • 2 University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China , Beijing (China)
  • 3 Chinese Academy of Sciences, Beijing Key Laboratory of Grape Sciences and Enology, CAS Key Laboratory of Plant Resources, Institute of Botany, Beijing, 100093, People’s Republic of China , Beijing (China)
Type
Published Article
Journal
Plant Molecular Biology
Publisher
Springer Netherlands
Publication Date
Apr 22, 2019
Volume
100
Issue
1-2
Pages
95–110
Identifiers
DOI: 10.1007/s11103-019-00846-6
Source
Springer Nature
Keywords
License
Yellow

Abstract

Key messageOverexpression of VaWRKY12, whose nuclear translocation increased under low temperature, enhanced the cold tolerance of Arabidopsis and grapevine calli and significantly increased the expression of antioxidant-related genes.AbstractLow temperature causes injuries to buds during winter and to young shoots during early spring, thereby affecting grapevine quality and yield. Understanding the regulatory mechanisms of cold stress responses is essential for the breeding of new grapevine cultivars with excellent cold tolerance. Previous studies indicated that WRKY family genes are induced by low temperature in grapevine, but their function in cold stress responses was not clear. Here, a cold-induced WRKY gene, named VaWRKY12, was cloned from Vitis amurensis, which displays remarkable cold tolerance. An atypical transmembrane (TM) region was found in its C-terminal region. Transient expression assays showed that VaWRKY12 was localized in the nucleus and cytoplasm at normal temperature but only in the nucleus after cold treatment. By contrast, a truncated version of VaWRKY12 without the TM region was found specifically in the nucleus at normal temperature, and its binding activity to tandem W-box elements in yeast was stronger than that of VaWRKY12, indicating that the TM region might affect the location and function of VaWRKY12. Overexpression of VaWRKY12 enhanced the cold tolerance of transformed Arabidopsis and grapevine calli. Transcriptome data revealed that the expression of genes encoding antioxidant enzymes, including peroxidases and glutathione S-transferases, was upregulated after cold treatment in VaWRKY12-overexpressing grapevine calli compared to the control calli. This study identifies candidate target genes as a basis for further studies on the roles of VaWRKY12 in cold stress responses in grapevine.

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