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Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus

Authors
  • Zhang, Jin1, 1
  • Xie, Meng1, 1, 2
  • Li, Mi2
  • Ding, Jinhua2, 3
  • Pu, Yunqiao1, 1
  • Bryan, Anthony C.1
  • Rottmann, William4
  • Winkeler, Kimberly A.4
  • Collins, Cassandra M.4
  • Singan, Vasanth5
  • Lindquist, Erika A.5
  • Jawdy, Sara S.1, 1
  • Gunter, Lee E.1, 1
  • Engle, Nancy L.1, 1
  • Yang, Xiaohan1, 1
  • Barry, Kerrie5
  • Tschaplinski, Timothy J.1, 1
  • Schmutz, Jeremy5, 6
  • Tuskan, Gerald A.1, 1
  • Muchero, Wellington1, 1
  • And 1 more
  • 1 Oak Ridge National Laboratory, USA , (United States)
  • 2 University of Tennessee, USA , (United States)
  • 3 Donghua University, China , (China)
  • 4 ArborGen Inc., USA , (United States)
  • 5 U.S. Department of Energy Joint Genome Institute, USA , (United States)
  • 6 HudsonAlpha Institute for Biotechnology, USA , (United States)
Type
Published Article
Journal
Plant Biotechnology Journal
Publisher
John Wiley and Sons Inc.
Publication Date
Sep 27, 2019
Volume
18
Issue
3
Pages
859–871
Identifiers
DOI: 10.1111/pbi.13254
PMID: 31498543
PMCID: PMC7004918
Source
PubMed Central
Keywords
License
Unknown
External links

Abstract

Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1‐6) form a jellyfish‐like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall‐related processes. Here, we report the functional characterization of a PFD gene from Populus deltoides , designated as PdPFD2.2 . There are two copies of PFD2 in Populus, and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2 , the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2 . A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2 . In addition, several known cell wall‐related TFs, such as MYB3 , MYB4 , MYB7 , TT8 and XND1 , were affected by overexpression of PdPFD2.2 . These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.

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