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Overexpression and initial characterization of the chromosomal aminoglycoside 3'-O-phosphotransferase APH(3')-IIb from Pseudomonas aeruginosa.

Authors
  • Hainrichson, Mariana
  • Yaniv, Orit
  • Cherniavsky, Marina
  • Nudelman, Igor
  • Shallom-Shezifi, Dalia
  • Yaron, Sima
  • Baasov, Timor
Type
Published Article
Journal
Antimicrobial agents and chemotherapy
Publication Date
Feb 01, 2007
Volume
51
Issue
2
Pages
774–776
Identifiers
PMID: 17088479
Source
Medline
License
Unknown

Abstract

The chromosomal gene aph(3')-IIb, encoding an aminoglycoside 3'-phosphotransferase in Pseudomonas aeruginosa, was cloned and overexpressed in Escherichia coli. The APH(3')-IIb enzyme was purified as a monomer in a two-step procedure and was shown to phosphorylate its substrates at the C-3'-OH position, with kcat/Km values of 0.4x10(4) to 36x10(4) M-1 s-1.

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