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Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells.

Authors
  • Levin, Aviad
  • Hayouka, Zvi
  • Friedler, Assaf
  • Loyter, Abraham
Type
Published Article
Journal
Virus Genes
Publisher
Springer-Verlag
Publication Date
Jun 01, 2010
Volume
40
Issue
3
Pages
341–346
Identifiers
DOI: 10.1007/s11262-010-0458-7
PMID: 20151187
Source
Medline
License
Unknown

Abstract

Expression of the human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for completion of the viral life cycle. Rev mediates nuclear export of partially spliced and unspliced viral transcripts and therefore bears a nuclear localization signal (NLS) as well as a nuclear export signal (NES), which allow its nucleocytoplasmic shuttling. Attempts to express the wild-type Rev protein in eukaryotic human cultured cells have encountered difficulties and so far have failed. Here we show that accumulation of Rev, which occurs in nondividing Rev-expressing cells or when such cells reach confluency, results in death of these cells. Cell death was also promoted by addition of a cell permeable peptide bearing the Rev-NES sequence, but not by the Rev-NLS peptide. Our results probably indicate that binding of excess amounts of the Rev protein or the NES peptide to the exportin receptor CRM1 results in cells' death.

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