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Osthole regulates inflammatory mediator expression through modulating NF-κB, mitogen-activated protein kinases, protein kinase C, and reactive oxygen species.

Authors
  • Liao, Pei-Chun
  • Chien, Shih-Chang
  • Ho, Chen-Lung
  • Wang, Eugene I-Chen
  • Lee, Shu-Ching
  • Kuo, Yueh-Hsiung
  • Jeyashoke, Narumon
  • Chen, Jie
  • Dong, Wei-Chih
  • Chao, Louis Kuoping
  • Hua, Kuo-Feng
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Oct 13, 2010
Volume
58
Issue
19
Pages
10445–10451
Identifiers
DOI: 10.1021/jf102812t
PMID: 20839800
Source
Medline
License
Unknown

Abstract

Osthole, a coumarin compound, has been reported to exhibit various biological activities; however the cellular mechanism of its immune modulating activity has not yet been fully addressed. In this study we isolated osthole from the seeds of Cnidium monnieri and demonstrated that osthole inhibited TNF-α, NO and COX-2 expression in LPS-stimulated macrophages, without reducing the expression of IL-6. Furthermore, the phosphorylation of p38, JNK1/2, PKC-α and PKC-ε induced by LPS was inhibited by osthole; however, the phosphorylation of ERK1/2 and PKC-δ was not reduced by osthole. Osthole also inhibited NF-κB activation and ROS release in LPS-stimulated macrophages. Our current results indicated that osthole is the major anti-inflammatory ingredient of Cnidium monnieri seed ethanol extract.

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