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OsERF2 controls rice root growth and hormone responses through tuning expression of key genes involved in hormone signaling and sucrose metabolism.

Authors
  • Xiao, Guiqing1, 2
  • Qin, Hua2
  • Zhou, Jiahao2
  • Quan, Ruidang2
  • Lu, Xiangyang3
  • Huang, Rongfeng4
  • Zhang, Haiwen5
  • 1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People's Republic of China. , (China)
  • 2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. , (China)
  • 3 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People's Republic of China. [email protected] , (China)
  • 4 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. [email protected] , (China)
  • 5 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. [email protected] , (China)
Type
Published Article
Journal
Plant molecular biology
Publication Date
Feb 01, 2016
Volume
90
Issue
3
Pages
293–302
Identifiers
DOI: 10.1007/s11103-015-0416-9
PMID: 26659593
Source
Medline
Keywords
License
Unknown

Abstract

Root determines plant distribution, development progresses, stress response, as well as crop qualities and yields, which is under the tight control of genetic programs and environmental stimuli. Ethylene responsive factor proteins (ERFs) play important roles in plant growth and development. Here, the regulatory function of OsERF2 involved in root growth was investigated using the gain-function mutant of OsERF2 (nsf2857) and the artificial microRNA-mediated silenced lines of OsERF2 (Ami-OsERF2). nsf2857 showed short primary roots compared with the wild type (WT), while the primary roots of Ami-OsERF2 lines were longer than those of WT. Consistent with this phenotype, several auxin/cytokinin responsive genes involved in root growth were downregulated in nsf2857, but upregulated in Ami-OsERF2. Then, we found that nsf2857 seedlings exhibited decreased ABA accumulation and sensitivity to ABA and reduced ethylene-mediated root inhibition, while those were the opposite in Ami-ERF2 plants. Moreover, several key genes involved in ABA synthesis were downregulated in nsf2857, but unregulated in Ami-ERF2 lines. In addition, OsERF2 affected the accumulation of sucrose and UDPG by mediating expression of key genes involved in sucrose metabolism. These results indicate that OsERF2 is required for the control of root architecture and ABA- and ethylene-response by tuning expression of series genes involved in sugar metabolism and hormone signaling pathways.

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