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Ornithine decarboxylase levels in patients with normal colonic mucosa.

Authors
  • Zehnter, E
  • Roisch, U
  • Kruis, W
  • Breuer, C
  • Diehl, V
Type
Published Article
Journal
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies
Publication Date
Jul 01, 1996
Volume
34
Issue
7
Pages
529–533
Identifiers
PMID: 8864401
Source
Medline
License
Unknown

Abstract

We describe a systematic examination of ornithine decarboxylase activity in 120 colonic mucosal samples which were obtained from 20 subjects without colonic disease to establish the normal mean and standard deviation from proximal to distal colon. Ornithine decarboxylase activity was determined by releasing CO2 from DL-[1-14C]ornithine. The mean ornithine decarboxylase levels (CO2 liberated) ranged from 0.26 +/- 0.08 nmol/h.mg protein in the caecum to 0.44 +/- 0.16 nmol/h.mg protein in the rectum. There was no difference between sex and age. Ornithine decarboxylase was not stimulated by guanosine 5'-triphosphate. alpha-Difluoromethylornithine showed an ornithine decarboxylase inhibition of 97.1%. Ornithine decarboxylase activity can be measured with reliable precision and reproducibility. The knowledge of the normal range of ornithine decarboxylase activity in normal human colonic mucosa is necessary for the determination of ornithine decarboxylase activity in pathological findings, especially in malignant transformation.

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