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Origins of sequence diversity in the malaria vaccine candidate merozoite surface protein-2 (MSP-2) in Amazonian isolates of Plasmodium falciparum.

Authors
  • Hoffmann, Erika H E
  • Malafronte, Rosely S
  • Moraes-Avila, Sandra L
  • Osakabe, Ana Lúcia
  • Wunderlich, Gerhard
  • Durham, Alan M
  • Ribolla, Paulo Eduardo M
  • del Portillo, Hernando A
  • Ferreira, Marcelo U
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Jul 19, 2006
Volume
376
Issue
2
Pages
224–230
Identifiers
PMID: 16716539
Source
Medline
License
Unknown

Abstract

The recent evolution of Plasmodium falciparum is at odds with the extensive polymorphism found in most genes coding for antigens. Here, we examined the patterns and putative mechanisms of sequence diversification in the merozoite surface protein-2 (MSP-2), a major malarial repetitive surface antigen. We compared the msp-2 gene sequences from closely related clones derived from sympatric parasite isolates from Brazilian Amazonia and used microsatellite typing to examine, in these same clones, the haplotype background of chromosome 2, where msp-2 is located. We found examples of msp-2 sequence rearrangements putatively created by nonreciprocal recombinational events, such as replication slippage and gene conversion, while maintaining the chromosome haplotype. We conclude that these nonreciprocal recombination events may represent a major source of antigenic diversity in MSP-2 in P. falciparum populations with low rates of classical meiotic recombination.

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