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Organization and transcription of canine (CAC)n sequences.

Authors
  • Hershfield, B
  • Inouye, L
  • Chader, G
  • Ripps, H
  • Aguirre, G
Type
Published Article
Journal
The Journal of heredity
Publication Date
Jan 01, 1991
Volume
82
Issue
3
Pages
251–254
Identifiers
PMID: 2061597
Source
Medline
License
Unknown

Abstract

Twenty-nine canine DNA samples, including samples from 11 members of an autosomal recessive retinal degeneration pedigree, were fingerprinted with a (CAC)n probe using a chemiluminescent method. Sixteen polymorphic bands were identified in the two pedigrees. Nine polymorphic bands were scored in one pedigree; seven polymorphic bands were scored in the other pedigree. None were sex-linked. Hybridization of the (CAC)n probe to total and poly A+ RNAs from different canine tissues revealed a prominent polyadenylated message at 6 kb that was present in the retina, spleen, and kidney but not in the liver or heart.

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