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Oral ingestion of Microbacterium nematophilum leads to anal-region infection in Caenorhabditis elegans.

Authors
  • Parsons, Lisa M1
  • Cipollo, John2
  • 1 Food and Drug Administration, Center for Biologics and Research, Building 29, 8800 Rockville Pike, Bethesda, MD 30892, USA.
  • 2 Food and Drug Administration, Center for Biologics and Research, Building 29, 8800 Rockville Pike, Bethesda, MD 30892, USA. Electronic address: [email protected]
Type
Published Article
Journal
Microbes and infection
Publication Date
Apr 01, 2014
Volume
16
Issue
4
Pages
356–361
Identifiers
DOI: 10.1016/j.micinf.2014.01.002
PMID: 24463271
Source
Medline
Keywords
License
Unknown

Abstract

Microbacterium nematophilum is a gram positive bacterium that colonizes the Caenorhabditis elegans rectal region causing swelling and constipation. This interaction has been exploited as a model system to identify and study genes important in host-pathogen interactions and innate immunity. During attempts to inhibit the host-pathogen interaction, it became important to clarify the route of infection. Using bacteria labeled with the fluorescent dye Cy3, we show that infection is via the oral route only and that infection follows a clear pattern of ingestion, plug formation, and bump development that can be quantitatively tracked over time.

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