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Optofluidic in situ maskless lithography of charge selective nanoporous hydrogel for DNA preconcentration.

Authors
  • Kim, Hyoki
  • Kim, Junhoi
  • Kim, Eun-Geun
  • Heinz, Austen James
  • Kwon, Sunghoon
  • Chun, Honggu
Type
Published Article
Journal
Biomicrofluidics
Publisher
American Institute of Physics
Publication Date
Dec 30, 2010
Volume
4
Issue
4
Pages
43014–43014
Identifiers
DOI: 10.1063/1.3516037
PMID: 21267091
Source
Medline
License
Unknown

Abstract

An optofluidic maskless photopolymerization process was developed for in situ negatively charged nanoporous hydrogel [poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] fabrication. The optofluidic maskless lithography system, which combines a high power UV source and digital mirror device, enables fast polymerization of arbitrary shaped hydrogels in a microfluidic device. The poly-AMPS hydrogel structures were positioned near the intersections of two microchannels, and were used as a cation-selective filter for biological sample preconcentration. Preconcentration dynamics as well as the fabricated polymer shape were analyzed in three-dimensions using fluorescein sample and a confocal microscope. Finally, single-stranded DNA preconcentration was demonstrated for polymerase chain reaction-free signal enhancement.

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