Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing.
- Authors
- Type
- Published Article
- Journal
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Volume
- 53
- Issue
- 7
- Pages
- 2329–2329
- Identifiers
- DOI: 10.1128/JCM.00956-15
- Source
- UCSC Bioengineering biomedical-ucsc
- License
- Unknown
Abstract
Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, which yielded high-quality sequence reads on the Illumina MiSeq platform.