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Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing.

Authors
Type
Published Article
Journal
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Volume
53
Issue
7
Pages
2329–2329
Identifiers
DOI: 10.1128/JCM.00956-15
Source
UCSC Bioengineering biomedical-ucsc
License
Unknown

Abstract

Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, which yielded high-quality sequence reads on the Illumina MiSeq platform.

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