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Optimization of Cultivation Conditions for the Lactobacillus reuteri LR1 Strain to Improve the Biosynthesis of Bacteriocin-Like Substances

Authors
  • Begunova, A. V.1
  • Rozhkova, I. V.1
  • Shirshova, T. I.1
  • Glazunova, O. A.2
  • Fedorova, T. V.2
  • 1 All-Russia Research Institute of Dairy Industry, Moscow, 115093, Russia , Moscow (Russia)
  • 2 Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia , Moscow (Russia)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
Dec 01, 2020
Volume
56
Issue
9
Pages
920–929
Identifiers
DOI: 10.1134/S0003683820090033
Source
Springer Nature
Keywords
License
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Abstract

The article presents the results of optimization of the culturing conditions to increase the production of bacteriocin-like compounds (presumably including reuterin) by the Lactobacillus reuteri strain LR1. The strain Escherichia coli ATCC 25922, an opportunistic microorganism, the content of which is standardized in foods by the Food Safety Legislation, was selected as an indicator strain of antimicrobial activity. The nutrient medium for L. reuteri LR1 cultivation was optimized to increase the production of bacteriocin-like compounds, and the parameters of two-stage growth of the producer were established: (1) cultivation on the initial medium for biomass accumulation and (2) growth on a water–glycerol medium to convert glycerol to antimicrobial compounds, presumably reuterin. The first stage was carried out as follows: the L. reuteri LR1 culture (5%) was inoculated into a medium with the following composition: hydrolyzed milk—250 mL/L; yeast extract—10 g/L; peptone—5 g/L; glucose—20 g/L; sodium acetate—5.0 g/L; monosubstituted potassium phosphate—2.0 g/L; magnesium sulfate—0.2 g/L, and manganese sulfate—0.5 g/L, pH 6.4–6.6. The cultivation was carried out for 18 h at (37 ± 1)°С. The second stage included the incubation of the obtained biomass in 200 mM of glycerol-containing aqueous solution, pH 6.6, for 2 h at 37°С. The zone of E. coli inhibition caused by the produced bacteriocin-like compounds was 25 mm.

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