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Optically modulated fluorophores for selective fluorescence signal recovery.

Authors
  • Richards, Chris I
  • Hsiang, Jung-Cheng
  • Senapati, Dulal
  • Patel, Sandeep
  • Yu, Junhua
  • Vosch, Tom
  • Dickson, Robert M
Type
Published Article
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Publication Date
Apr 08, 2009
Volume
131
Issue
13
Pages
4619–4621
Identifiers
DOI: 10.1021/ja809785s
PMID: 19284790
Source
Medline
License
Unknown

Abstract

Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.

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