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Optical trapping and manipulation of live T cells with a low numerical aperture lens.

Authors
  • Harris, John1
  • McConnell, Gail
  • 1 Centre for Biophotonics, Strathclyde Institute for Pharmacy and Biomedical Sciences, University Of Strathclyde, 27 Taylor St, Glasgow G4 0NR, United Kingdom. [email protected] , (United Kingdom)
Type
Published Article
Journal
Optics Express
Publisher
The Optical Society
Publication Date
Sep 01, 2008
Volume
16
Issue
18
Pages
14036–14043
Identifiers
PMID: 18773014
Source
Medline
License
Unknown

Abstract

An optical manipulation system that employs both optical and temperature gradients to simultaneously enable trapping, manipulating and imaging of live cells with a low magnification, low numerical aperture objective lens (10x/0.4 N.A.) is reported. This approach negates the requirement for a high N.A. lens used in traditional optical trapping. Our system comprised a dual scanning system and two independent lasers which provided the ability to move the trapping spot independently of the confocal imaging process in close to real-time and without pre-programming. To demonstrate the efficacy of this innovative method, trapping and manipulation of live T cells was simultaneously performed over a field of view exceeding 1 mm(2) for extended periods without compromising cell viability.

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