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Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases.

Authors
  • Michaeli, Yael1
  • Shahal, Tamar
  • Torchinsky, Dmitry
  • Grunwald, Assaf
  • Hoch, Rony
  • Ebenstein, Yuval
  • 1 Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv, Israel. [email protected] , (Israel)
Type
Published Article
Journal
Chemical Communications
Publisher
The Royal Society of Chemistry
Publication Date
Oct 07, 2013
Volume
49
Issue
77
Pages
8599–8601
Identifiers
DOI: 10.1039/c3cc42543f
PMID: 23756466
Source
Medline
License
Unknown

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

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