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Optical ammonia sensors based on fluorescent aza-BODIPY dyes- a flexible toolbox.

Authors
  • Maierhofer, Maximilian1
  • Rieger, Veronika1
  • Mayr, Torsten2
  • 1 Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/2, 8010, Graz, Austria. , (Austria)
  • 2 Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology, Stremayrgasse 9/2, 8010, Graz, Austria. [email protected] , (Austria)
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
Nov 01, 2020
Volume
412
Issue
27
Pages
7559–7567
Identifiers
DOI: 10.1007/s00216-020-02891-3
PMID: 32839858
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We present three types of optical ammonia sensors suitable for environmental, bioprocess, and reaction monitoring. A respective fluorescent BF2-chelated tetraarylazadipyrromethene dye (aza-BODIPYs) is physically entrapped in a polyurethane hydrogel (HydroMed D4) forming an emulsion system with vinyl-terminated polydimethylsiloxane (PDMS). The analyte-sensitive layer is covered by a hydrophobic membrane which excludes hydrophilic substances. Three different protection layers are tested, whereby the Teflon and the hydrophobic PES layers outperform a PDMS/TiO2 layer. Response times within their dynamic range of 15 s can be achieved, whereas the PDMS/TiO2-covered sensor requires at least 390 s. The three sensors entail the following concentration areas: first sensor 3 μg L-1-3 mg L-1 (LOD 0.23 μg L-1), second sensor 0.1-30 mg L-1 (LOD 28 μg L-1), and third sensor 3 mg L-1-1 g L-1 (LOD 0.51 mg L-1). Readout is performed with a commercially available phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) is used as referencing method and Egyptian blue acts as an inert reference material. No cross-sensitivity to pH changes can be detected.

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