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Opposing Roles of Dopamine Receptor D1- and D2-Expressing Neurons in the Anteromedial Olfactory Tubercle in Acquisition of Place Preference in Mice

Authors
  • Murata, Koshi1, 2
  • Kinoshita, Tomoki1
  • Fukazawa, Yugo1, 2, 3
  • Kobayashi, Kenta4
  • Yamanaka, Akihiro5
  • Hikida, Takatoshi6
  • Manabe, Hiroyuki7
  • Yamaguchi, Masahiro8
  • 1 Division of Brain Structure and Function, Faculty of Medical Sciences, University of Fukui, Fukui , (Japan)
  • 2 Life Science Innovation Center, Faculty of Medical Science, University of Fukui, Fukui , (Japan)
  • 3 Research Center for Child Mental Health Development, Faculty of Medical Sciences, University of Fukui, Fukui , (Japan)
  • 4 Section of Viral Vector Development, National Institute for Physiological Sciences, Aichi , (Japan)
  • 5 Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Aichi , (Japan)
  • 6 Laboratory for Advanced Brain Functions, Institute for Protein Research, Osaka University, Osaka , (Japan)
  • 7 Laboratory of Neural Information, Graduate School of Brain Science, Doshisha University, Kyoto , (Japan)
  • 8 Department of Physiology, Kochi Medical School, Kochi University, Kochi , (Japan)
Type
Published Article
Journal
Frontiers in Behavioral Neuroscience
Publisher
Frontiers Media SA
Publication Date
Mar 15, 2019
Volume
13
Identifiers
DOI: 10.3389/fnbeh.2019.00050
Source
Frontiers
Keywords
Disciplines
  • Neuroscience
  • Original Research
License
Green

Abstract

Olfaction induces adaptive motivated behaviors. Odors associated with food induce attractive behavior, whereas those associated with dangers induce aversive behavior. We previously reported that learned odor-induced attractive and aversive behaviors accompany activation of the olfactory tubercle (OT) in a domain- and cell type-specific manner. Odor cues associated with a sugar reward induced attractive behavior and c-fos expression in the dopamine receptor D1-expressing neurons (D1 neurons) in the anteromedial OT. In contrast, odor cues associated with electrical shock induced aversive behavior and c-fos expression in the pamine receptor D2-expressing neurons (D2 neurons) in the anteromedial OT, as well as the D1 neurons in the lateral OT. Here, we investigated whether the D1 and D2 neurons in the anteromedial OT play distinct roles in attractive or aversive behaviors, using optogenetic stimulation and real-time place preference (RTPP) tests. Mice expressing ChETA (ChR2/E123T)-enhanced yellow fluorescent protein (EYFP) in the D1 neurons in the anteromedial OT spent a longer time in the photo-stimulation side of the place preference chamber than the control mice expressing EYFP. On the other hand, upon optogenetic stimulation of the D2 neurons in the anteromedial OT, the mice spent a shorter time in the photo-stimulation side than the control mice. Local neural activation in the anteromedial OT during the RTPP tests was confirmed by c-fos mRNA expression. These results suggest that the D1 and D2 neurons in the anteromedial OT play opposing roles in attractive and aversive behaviors, respectively.

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