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Theoretical Analysis of Efficiency of Multi-Layer Core-Shell Stationary Phases in the High Performance Liquid Chromatography of Large Biomolecules.

Authors
  • Horváth, Szabolcs1
  • Gritti, Fabrice2
  • Kormány, Róbert1, 3
  • Horváth, Krisztián4
  • 1 Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary. , (Hungary)
  • 2 Waters Corporation, 34 Maple Street, Milford, MA 01757, USA.
  • 3 Egis Pharmaceuticals PLC, Keresztúri út 30-38, 1106 Budapest, Hungary. , (Hungary)
  • 4 Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary. [email protected] , (Hungary)
Type
Published Article
Journal
Molecules
Publisher
MDPI AG
Publication Date
Aug 06, 2019
Volume
24
Issue
15
Identifiers
DOI: 10.3390/molecules24152849
PMID: 31390734
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Modern analytical applications of liquid chromatography require columns with higher and higher efficiencies. In this work, the general rate model (GRM) of chromatography is used for the analysis of the efficiency of core-shell phases having two porous layers with different structures and/or surface chemistries. The solution of the GRM in the Laplace domain allows for the calculation of moments of elution curves (retention time and peak width), which are used for the analysis of the efficiency of bi-layer particles with and without a non-porous core. The results demonstrate that bi-layer structures can offer higher separation power than that of the two layers alone if the inner layer has smaller surface coverage (retentivity) and the pore size and pore diffusion of the outer layer is either equal to or higher than that of the inner layer. Even in the case of core-shell phases, there is an increase in resolution by applying the bi-layer structure; however, we can always find a mono-layer core-shell particle structure with a larger core size that provides better resolution. At the optimal core size, the resolution cannot be further improved by applying a bi-layer structure. However, in case of the most widely produced general-purpose core-shell particles, where the core is ∼70% of the particle diameter, a 15-20% gain of resolution can be obtained by using well-designed and optimized bi-layer core-shell phases.

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