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Online preconcentration by transient isotachophoresis in linear polymer on a poly(methyl methacrylate) microchip for separation of human serum albumin immunoassay mixtures.

Authors
  • Mohamadi, Mohamad Reza
  • Kaji, Noritada
  • Tokeshi, Manabu
  • Baba, Yoshinobu
Type
Published Article
Journal
Analytical chemistry
Publication Date
May 15, 2007
Volume
79
Issue
10
Pages
3667–3672
Identifiers
PMID: 17437335
Source
Medline
License
Unknown

Abstract

Online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody by on-chip transient isotachophoresis is reported. An 800-fold signal enhancement was achieved following the preconcentration on standard cross-channel microchips made of poly (methyl methacrylate). Sample injection, preconcentration, and separation were done continuously and controlled solely by a sequential voltage switching program. The preconcentration was followed by on-chip nondenaturing gel electrophoresis in methylcellulose solution. The method was applied to microchip electrophoresis immunoassay of HSA. Baseline separation of HSA and its immunocomplex was achieved in 25 s in the first 1 cm of the microchannel. In a direct immunoassay, the minimum detectable concentration of fluorescent labeled HSA by laser-induced fluorescence detection was 7.5 pM.

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