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Oligonucleotides with 1,5-anhydrohexitol nucleoside building blocks: crystallization and preliminary X-ray studies of h(GTGTACAC).

Authors
  • Declercq, R
  • Van Aerschot, A
  • Herdewijn, P
  • Van Meervelt, L
Type
Published Article
Journal
Acta crystallographica. Section D, Biological crystallography
Publication Date
Jan 01, 1999
Volume
55
Issue
Pt 1
Pages
279–280
Identifiers
PMID: 10089424
Source
Medline
License
Unknown

Abstract

Hexitol nucleic acids are oligonucleotides built up from natural nucleobases and a phosphorylated 1,5-anhydrohexitol backbone. The anhydrohexitol oligonucleotide h(GTGTACAC) was synthesized using phosphoramidite chemistry and standard protecting groups. Crystals of h(GTGTACAC) were obtained at either 279 or 289 K by the hanging-drop vapour-diffusion technique using a 24-matrix screen for nucleic acid fragments. The crystals diffract beyond 2.0 A resolution and belong to the hexagonal space group P6222 (or P6422) with unit-cell parameters a = 36.42 and c = 63.33 A.

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