Affordable Access

deepdyve-link
Publisher Website

Oligonucleotide delivery by a cationic derivative of the polyene antibiotic amphotericin B

Authors
  • Blanc, Isabelle
  • Saint-Pierre Chazalet, Michèle
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier
Publication Date
Jan 01, 2000
Accepted Date
Jan 31, 2000
Volume
1464
Issue
2
Pages
309–321
Identifiers
DOI: 10.1016/S0005-2736(00)00161-9
Source
Elsevier
Keywords
License
Unknown

Abstract

We report a study of the behavior of oligodeoxyribonucleotide (ODN)/amphotericin B3-( N′-dimethylamino)propylamide (AMA) complexes, in the presence of lipid monolayers and large unilamellar vesicles. This study follows the recent discovery of the capacity of AMA, as a new cationic vector, to enhance ODN cellular uptake and efficacy. It aims at investigating the internalization mode of a nucleic acid by AMA. A first study at the air–water interface of AMA and AMA/ODN by surface pressure measurement shows that only free AMA would adsorb at the air–water interface. Second, in the presence of zwitterionic phospholipid- and sterol-containing mixture, ODN–AMA interactions in solution would be higher than lipid–AMA interactions at the interface. In monolayer or with large unilamellar vesicles, AMA monomers adsorb mainly at the phospholipid interface. These results favor a crossing mechanism through AMA channel formation, despite the size of ODN.

Report this publication

Statistics

Seen <100 times