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OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage

Authors
  • Miglani, Kanika1
  • Kumar, Sunil2
  • Yadav, Anita2
  • Aggarwal, Neeraj3
  • Gupta, Ranjan1
  • 1 Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, India;
  • 2 Department of Biotechnology, Kurukshetra University Kurukshrtra, Haryana 136119, India;
  • 3 Department of Microbiology, Kurukshetra University Kurukshetra, Haryana 136119, India
Type
Published Article
Journal
Iranian Biomedical Journal
Publisher
Pasteur Institute of Iran
Publication Date
Aug 31, 2020
Volume
25
Issue
1
Pages
47–53
Identifiers
DOI: 10.29252/ibj.25.1.47
PMID: 33129239
PMCID: PMC7748119
Source
PubMed Central
Keywords
Disciplines
  • Full Length
License
Green

Abstract

Background: Single nucleotide polymorphisms in OGG1 gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-OHdG mutagenicity. OGG1 -Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this study was to examine the link of oxidative and genotoxic DNA damage with DNA repair OGG1 gene polymorphism, in charcoal workers exposed to polyaromatic hydrocarbons. Methods: Urinary 8-OHdG excretion (a biomarker of oxidative DNA damage) was determined in both exposed and control populations. Genotyping of OGG1 DNA repair gene in the blood samples of subjects was carried out by PCR-RFLP method. Results: The 8-OHdG urinary concentration was significantly higher ( p < 0.05) in the exposed (geometric mean 12.33 ± 3.78) than in the unexposed (geometric mean 7.36 ± 2.29) population. DNA damage, as measured by 8-OHdG and TM content, was found to be significantly higher in OGG1 homozygous mutants (mt/mt; 18.81 ± 3.34; 6.04 ± 0.52) as compared to wild-type genotypes (wt/wt; 10.34 ± 2.25; 5.19 ± 2.50) and heterozygous (wt/mt) mutants (12.82 ± 2.81; 6.04 ± 0.93) in the exposed group. Conclusion: We found a significant association of OGG1 heterozygous ( wt/mt ) and homozygous ( mt/mt ) variants with oxidative and genotoxic damage, suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational workers.

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